Journal: Nature methods

Article Title: Miniaturized integration of a fluorescence microscope

doi: 10.1038/nmeth.1694

Figure Lengend Snippet: a . Computer-assisted design of an integrated microscope, shown in cross-section. Blue and green arrows mark illumination and emission pathways, respectively. Scale bar is 5 mm. b . Assembled integrated microscope. Insets show, clockwise from bottom left: filter cube holding dichroic mirror and excitation and emission filters; printed circuit board (PCB) holding the complementary metal-oxide-semiconductor (CMOS) camera chip; PCB holding the light emitting diode (LED) illumination source. The wire bundles for LED and CMOS boards are visible. Scale bar is 5 mm and applies to all four photographs. c . Electronics for real-time image acquisition and control. The LED and CMOS sensoreach have their own PCB. These boards are connected to a custom, external PCB via nine fine wires (two to the LED and seven to the camera) encased in a single polyvinyl chloride sheath. The external PCB interfaces with a computer via a USB adaptor board. Labeled components summarize circuitry for providing power, controlling the LED and CMOS camera, and transferring data. Abbreviations: PD, flash programming device; OSC, Quartz crystal oscillator; I 2 C, Two-wire Inter-Integrated Circuit serial communication interface.

Article Snippet: To record mouse behavior, we used either a video rate monochrome CMOS camera (Prosilica, EC640) with a high-resolution lens (Computar, M0814-MP2) situated above the arena or a video rate color CCD camera (Sony, DCR-VX2000NTSC) placed adjacent to the arena.

Techniques: Microscopy, Control, Labeling, Transferring